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tecan spark luminometer  (Tecan Systems)


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    Structured Review

    Tecan Systems tecan spark luminometer
    Tecan Spark Luminometer, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 17117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tecan+spark+luminometer/Spark/pm41750373-67-15-15
    Average 99 stars, based on 17117 article reviews
    tecan spark luminometer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Structure-Aided Design of a LuxR-Type Quorum Sensing SuFEx-Based Potential Inhibitor: Covalent or Competitive Inhibition?
    Article Snippet: .. Acyl-HSL activity was measured in a 96-well microtiter plate format, with bioluminescence quantified using a TECAN Spark luminometer. ..

    Article Title: Carboxylic Acid Bioisosteres Boost Nurr1 Agonist Selectivity.
    Article Snippet: After 24 h, medium was changed to Opti-MEM without supplements and cells were transiently transfected using Lipofectamine LTX reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. Five hours after transfection, cells were incubated with the test compounds in Opti-MEM supplemented with penicillin (100 U/mL), streptomycin (100 μg/ mL) and 0.1% DMSO for 16 h before luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer’s protocol on a Tecan Spark luminometer (Tecan Deutschland GmbH, Crailsheim, Germany). ..

    Article Title: A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice
    Article Snippet: .. Luciferase activity was detected using 100 μl/well of Luciferase Assay Reagent (Promega), and emitted light was immediately quantified using a Tecan Spark luminometer. ..

    Article Title: Fragment-based discovery of dual ligand pharmacophores for lipid-sensing transcription factors for designed polypharmacology
    Article Snippet: After 24 h, medium was changed to Opti-MEM without supplements and cells were transiently transfected using Lipofectamine LTX reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. .. Five hours after transfection, cells were incubated with the test compounds in Opti-MEM supplemented with penicillin (100 U mL −1 ), streptomycin (100 μg mL −1 ) and 0.1% DMSO for 16 h before luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer's protocol on a Tecan Spark luminometer (Tecan Deutschland GmbH, Crailsheim, Germany). ..

    Article Title: Structure-Aided Design of a LuxR-Type Quorum Sensing SuFEx-Based Potential Inhibitor: Covalent or Competitive Inhibition?
    Article Snippet: .. Acyl-HSL activity was measured in a 96-well microtiter plate format, with bioluminescence quantified using a TECAN Spark luminometer. ..

    other:

    Article Title: A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice.
    Article Snippet: Luciferase assay with hTLR4-transfected HEK293 NF-κBreporter cell lines HEK293-hTLR4-transfected adherent cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 4.5 g/l glucose and HEPES (GIBCO), 10% fetal bovine serum (FBS, Hyclone), 1% penicillin/ streptomycin/glutamine, and specific antibiotics for the different cell lines: puromycin (5 μg/ml Invivogen), blasticidin (10 μg/ml; Invivogen), hygromycin (250 μg/ml, Roche).

    Infection:

    Article Title: Evolutionary characterization of antiviral SAMD9/9L across kingdoms supports ancient convergence and lineage-specific adaptations.
    Article Snippet: .. Following 48 h of infection, cell lysis was performed using BrightGlow Lysis Reagent (Promega E2620) and relative light units were measured using the Tecan Spark Luminometer. ..

    Article Title: Evolutionary characterization of antiviral SAMD9/9L across kingdoms supports ancient convergence and lineage-specific adaptations
    Article Snippet: .. Following 48 h of infection, cell lysis was performed using BrightGlow Lysis Reagent (Promega E2620) and relative light units were measured using the Tecan Spark Luminometer. ..

    Lysis:

    Article Title: Evolutionary characterization of antiviral SAMD9/9L across kingdoms supports ancient convergence and lineage-specific adaptations.
    Article Snippet: .. Following 48 h of infection, cell lysis was performed using BrightGlow Lysis Reagent (Promega E2620) and relative light units were measured using the Tecan Spark Luminometer. ..

    Article Title: Evolutionary characterization of antiviral SAMD9/9L across kingdoms supports ancient convergence and lineage-specific adaptations
    Article Snippet: .. Following 48 h of infection, cell lysis was performed using BrightGlow Lysis Reagent (Promega E2620) and relative light units were measured using the Tecan Spark Luminometer. ..

    Transfection:

    Article Title: Carboxylic Acid Bioisosteres Boost Nurr1 Agonist Selectivity.
    Article Snippet: After 24 h, medium was changed to Opti-MEM without supplements and cells were transiently transfected using Lipofectamine LTX reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. Five hours after transfection, cells were incubated with the test compounds in Opti-MEM supplemented with penicillin (100 U/mL), streptomycin (100 μg/ mL) and 0.1% DMSO for 16 h before luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer’s protocol on a Tecan Spark luminometer (Tecan Deutschland GmbH, Crailsheim, Germany). ..

    Article Title: Fragment-based discovery of dual ligand pharmacophores for lipid-sensing transcription factors for designed polypharmacology
    Article Snippet: After 24 h, medium was changed to Opti-MEM without supplements and cells were transiently transfected using Lipofectamine LTX reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. .. Five hours after transfection, cells were incubated with the test compounds in Opti-MEM supplemented with penicillin (100 U mL −1 ), streptomycin (100 μg mL −1 ) and 0.1% DMSO for 16 h before luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer's protocol on a Tecan Spark luminometer (Tecan Deutschland GmbH, Crailsheim, Germany). ..

    Incubation:

    Article Title: Carboxylic Acid Bioisosteres Boost Nurr1 Agonist Selectivity.
    Article Snippet: After 24 h, medium was changed to Opti-MEM without supplements and cells were transiently transfected using Lipofectamine LTX reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. Five hours after transfection, cells were incubated with the test compounds in Opti-MEM supplemented with penicillin (100 U/mL), streptomycin (100 μg/ mL) and 0.1% DMSO for 16 h before luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer’s protocol on a Tecan Spark luminometer (Tecan Deutschland GmbH, Crailsheim, Germany). ..

    Article Title: Fragment-based discovery of dual ligand pharmacophores for lipid-sensing transcription factors for designed polypharmacology
    Article Snippet: After 24 h, medium was changed to Opti-MEM without supplements and cells were transiently transfected using Lipofectamine LTX reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. .. Five hours after transfection, cells were incubated with the test compounds in Opti-MEM supplemented with penicillin (100 U mL −1 ), streptomycin (100 μg mL −1 ) and 0.1% DMSO for 16 h before luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer's protocol on a Tecan Spark luminometer (Tecan Deutschland GmbH, Crailsheim, Germany). ..

    Luciferase:

    Article Title: Carboxylic Acid Bioisosteres Boost Nurr1 Agonist Selectivity.
    Article Snippet: After 24 h, medium was changed to Opti-MEM without supplements and cells were transiently transfected using Lipofectamine LTX reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. Five hours after transfection, cells were incubated with the test compounds in Opti-MEM supplemented with penicillin (100 U/mL), streptomycin (100 μg/ mL) and 0.1% DMSO for 16 h before luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer’s protocol on a Tecan Spark luminometer (Tecan Deutschland GmbH, Crailsheim, Germany). ..

    Article Title: A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice
    Article Snippet: .. Luciferase activity was detected using 100 μl/well of Luciferase Assay Reagent (Promega), and emitted light was immediately quantified using a Tecan Spark luminometer. ..

    Article Title: Fragment-based discovery of dual ligand pharmacophores for lipid-sensing transcription factors for designed polypharmacology
    Article Snippet: After 24 h, medium was changed to Opti-MEM without supplements and cells were transiently transfected using Lipofectamine LTX reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. .. Five hours after transfection, cells were incubated with the test compounds in Opti-MEM supplemented with penicillin (100 U mL −1 ), streptomycin (100 μg mL −1 ) and 0.1% DMSO for 16 h before luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer's protocol on a Tecan Spark luminometer (Tecan Deutschland GmbH, Crailsheim, Germany). ..



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